• Isolating DNA fragments: Method 1 using reverse transcriptase, method 2 using restriction endonucleases
  • Method 3 - the gene machine; amplifying DNA fragments in vivo [Inserting DNA fragments into vector; transforming cells]
  • identification of transformed cells; Marker genes; Antibiotic resistance gene markers; fluorescent markers
  • In vitro gene cloning PCR: separation of DNA strand; addition (annealing) of primers; synthesis of DNA
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Recombinant DNA Technology

Recombinant DNA Technology

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Isolating DNA fragments: Method 1 - using reverse transcriptase, method 2 -  using restriction endonucleases

Method 3 - the gene machine; amplifying DNA fragments in vivo [Inserting DNA fragments into vector; transforming cells]

identification of transformed cells; Marker genes; Antibiotic resistance gene markers; fluorescent markers

In vitro gene cloning PCR: separation of DNA strand; addition  (annealing) of primers; synthesis of DNA

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